Ītoui A, Mansouria A, Boskoub G, Kefal P (2005) Tea and herbal infusions: their antioxidant activity and phenolic profile. Leaves Indian drugs 49:13–18Īo C, Li A, Elzaawely AA, Xuan TD, Tawata S (2008) Evaluation of antioxidant and antibacterial activities of Ficus microcarpa L. Īli A, Ali M (2012) New fatty acid derivatives from Ocimum sanctum L. sanctum leaf extracts in butanol and ethylacetate with high polyphenolics and flavonoids, had strong antioxidant potential.Īgarwal K, Singh DK, Jyotshna J, Ahmad A, Shanker K, Tandon S, Luqman S (2017) Antioxidative potential of two chemically characterized Ocimum (Tulsi) species extracts. Quercetin is extracted in ethylacetate fraction. LC–MS analysis of OsB and OsE showed the presence of luteolin, apigenin, rosmarinic, chlorogenic, caffeic acid and their derivatives. EC 50 values of different fractions for DPPH anion, ABTS cation free radical scavenging and phosphomolybdate reducing property were significantly and positively correlated with TPC and TFC.
OsE also had significantly high TPC (202.71 ± 5.5 mg GAE/g extract).
OsB was rich in both total polyphenolic content (TPC) (212.26 ± 6.3 mg GAE/g extract) and total flavonoid contents (TFC) (54.51 ± 3.5 mg QE/g extract). The leaves of Ocimum sanctum were extracted in methanol (OsM) and sequentially fractionated with n-hexane (OsH), ethylacetate (OsE) and butanol (OsB) to find the best extraction solvent for antioxidants from the herb known for its medicinal values.
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